The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add. Find prestained ladders compatible for western blotting. Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Note: Unstained / Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Staining Reagent / Coomassie Blue Categories: Protein Marker/Ladder. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. Find the right protein ladder (molecular weight markers) for your protein electrophoresis needs. UNveil Unstained Protein Ladder10 to 200 kDa Size: 500 l. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. For size determination on western blots, depending on the visualization method being used, the protein ladder can be unstained, prestained, or with a Strep -tag sequence. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.Īb116027 has been referenced in 5 publications. Introduction to Western Blot Protein Standards. Review other protein ladders in the unstained and prestained protein ladder guide. Recommended loading: ~2 - 3 µl for western blots.Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The ladder is supplied in gel loading buffer and is ready to use.ĭo not heat, dilute, add reducing agent before loading. This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. New England Biolabs offers a selection of highly pure protein standards.